protocol-book
  • Salis Lab Protocol Book
  • Getting Started
  • Modeling & Algorithms
    • Linux
      • Install
      • Using Bash
      • CRUD Files & Directories
      • Text Editors
      • Git
    • Server Computing
      • ssh
      • High Performance Computing
      • Cloud Computing
    • Dev
      • How to be a Pythonista
      • Developing Python Packages
      • Setting up a repo
    • Bioinformatics Tools
      • BLAST
      • BWA-mem
      • kallisto
      • mfold
      • OligoAnalyzer
      • OligoCalc
      • ViennaRNA
    • DNA Sequence Editors
      • A Plasmid Editor
      • Benchling
    • Online Resources
      • Addgene
      • CGSC
      • EcoCyc
      • Genbank (NCBI)
      • RegulonDB
  • Molecular Biology
    • Microbiology Lab
      • Personal Protective Equipment
      • Aseptic Technique
      • Media & Recipes
      • Growing Bacteria
      • Enumerating Bacteria
      • Storing Bacteria
      • Bacteriophages
    • Lab Equipment
      • Shaker Incubators
      • Genetic QPix2 Colony Picker
      • NanoDrop
      • Thermal Cyclers
      • Microplate Reader
      • Microplate Incubator
  • Design Genetic Systems
    • Models and Design Algorithms
      • Getting started
      • RBS Calculator
      • RBS Library Calculator
      • Riboswitch Calculator
      • Operon Calculator
      • Non-Repetitive Parts Calculator
      • Synthesis Success Calculator
      • PyVRNA
      • RNAdynamics
      • Dependencies
      • Glossary
      • UIPAC Code
      • Jupyter tutorials
  • Build Genetic Systems
    • Plan and Execute Your DOE
    • DNA
      • Ordering DNA
      • DNA Assembly
      • Anneal Oligonucleotides
      • PCR
      • PCR Cleanup
      • PCR Assembly
      • Gel Extraction
      • Plasmid Extraction
      • Genomic Library Preparation
    • Restriction Cloning
      • Restriction Enzyme Digestion
      • Ligation with T4 DNA Ligase
    • Multi-Fragment Assembly
      • Golden Gate
      • LCR
      • Gibson Assembly
      • TA Cloning
    • Genome Editing
      • Lambda Red Recombination
      • MAGE
      • pORTMAGE
      • CRISPR/Cas9
    • Transformation and Integration
      • E. coli Electroporation
      • E. coli Heat Shock
      • B. subtilis
  • Test
    • DNA
      • Gel Electrophoresis
      • Sanger Sequencing
      • Illumina
      • LCR-Seq
    • Protein
      • Flow Cytometry
      • Fluorescence Activated Cell Sorting
      • Gram's Iodine Stain
      • LacZ (beta-gal)
      • Microplate Fluoresence
      • Neurosporene
      • SDS-PAGE
      • TX-TL Crude Cell Extract Prep
      • Transcription-Translation (TX-TL)
    • RNA
      • Spin Column-Based Extraction
      • Phenol-Chloroform Extraction
      • Reverse Transcriptase (RT)
      • qPCR with TaqMan
      • qPCR with SYBR Green
      • Targeted RNA-Seq
      • Transcriptome RNA-Seq
      • T7 HiScribe Kit RNA Synthesis
      • T7 RNAP Reaction Clean-Up
    • Metabolite
      • HPLC
      • LC-MS
    • Other
      • TCSPC
      • MinION
Powered by GitBook
On this page
  1. Build Genetic Systems

Multi-Fragment Assembly

PreviousLigation with T4 DNA LigaseNextGolden Gate

Last updated 6 years ago

Golden Gate
LCR
Gibson Assembly
TA Cloning