protocol-book
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    • Bioinformatics Tools
      • BLAST
      • BWA-mem
      • kallisto
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    • DNA Sequence Editors
      • A Plasmid Editor
      • Benchling
    • Online Resources
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  • Molecular Biology
    • Microbiology Lab
      • Personal Protective Equipment
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      • Media & Recipes
      • Growing Bacteria
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  • Design Genetic Systems
    • Models and Design Algorithms
      • Getting started
      • RBS Calculator
      • RBS Library Calculator
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      • Synthesis Success Calculator
      • PyVRNA
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      • UIPAC Code
      • Jupyter tutorials
  • Build Genetic Systems
    • Plan and Execute Your DOE
    • DNA
      • Ordering DNA
      • DNA Assembly
      • Anneal Oligonucleotides
      • PCR
      • PCR Cleanup
      • PCR Assembly
      • Gel Extraction
      • Plasmid Extraction
      • Genomic Library Preparation
    • Restriction Cloning
      • Restriction Enzyme Digestion
      • Ligation with T4 DNA Ligase
    • Multi-Fragment Assembly
      • Golden Gate
      • LCR
      • Gibson Assembly
      • TA Cloning
    • Genome Editing
      • Lambda Red Recombination
      • MAGE
      • pORTMAGE
      • CRISPR/Cas9
    • Transformation and Integration
      • E. coli Electroporation
      • E. coli Heat Shock
      • B. subtilis
  • Test
    • DNA
      • Gel Electrophoresis
      • Sanger Sequencing
      • Illumina
      • LCR-Seq
    • Protein
      • Flow Cytometry
      • Fluorescence Activated Cell Sorting
      • Gram's Iodine Stain
      • LacZ (beta-gal)
      • Microplate Fluoresence
      • Neurosporene
      • SDS-PAGE
      • TX-TL Crude Cell Extract Prep
      • Transcription-Translation (TX-TL)
    • RNA
      • Spin Column-Based Extraction
      • Phenol-Chloroform Extraction
      • Reverse Transcriptase (RT)
      • qPCR with TaqMan
      • qPCR with SYBR Green
      • Targeted RNA-Seq
      • Transcriptome RNA-Seq
      • T7 HiScribe Kit RNA Synthesis
      • T7 RNAP Reaction Clean-Up
    • Metabolite
      • HPLC
      • LC-MS
    • Other
      • TCSPC
      • MinION
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  1. Modeling & Algorithms

Online Resources

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Last updated 6 years ago

Addgene
CGSC
EcoCyc
Genbank (NCBI)
RegulonDB