protocol-book
  • Salis Lab Protocol Book
  • Getting Started
  • Modeling & Algorithms
    • Linux
      • Install
      • Using Bash
      • CRUD Files & Directories
      • Text Editors
      • Git
    • Server Computing
      • ssh
      • High Performance Computing
      • Cloud Computing
    • Dev
      • How to be a Pythonista
      • Developing Python Packages
      • Setting up a repo
    • Bioinformatics Tools
      • BLAST
      • BWA-mem
      • kallisto
      • mfold
      • OligoAnalyzer
      • OligoCalc
      • ViennaRNA
    • DNA Sequence Editors
      • A Plasmid Editor
      • Benchling
    • Online Resources
      • Addgene
      • CGSC
      • EcoCyc
      • Genbank (NCBI)
      • RegulonDB
  • Molecular Biology
    • Microbiology Lab
      • Personal Protective Equipment
      • Aseptic Technique
      • Media & Recipes
      • Growing Bacteria
      • Enumerating Bacteria
      • Storing Bacteria
      • Bacteriophages
    • Lab Equipment
      • Shaker Incubators
      • Genetic QPix2 Colony Picker
      • NanoDrop
      • Thermal Cyclers
      • Microplate Reader
      • Microplate Incubator
  • Design Genetic Systems
    • Models and Design Algorithms
      • Getting started
      • RBS Calculator
      • RBS Library Calculator
      • Riboswitch Calculator
      • Operon Calculator
      • Non-Repetitive Parts Calculator
      • Synthesis Success Calculator
      • PyVRNA
      • RNAdynamics
      • Dependencies
      • Glossary
      • UIPAC Code
      • Jupyter tutorials
  • Build Genetic Systems
    • Plan and Execute Your DOE
    • DNA
      • Ordering DNA
      • DNA Assembly
      • Anneal Oligonucleotides
      • PCR
      • PCR Cleanup
      • PCR Assembly
      • Gel Extraction
      • Plasmid Extraction
      • Genomic Library Preparation
    • Restriction Cloning
      • Restriction Enzyme Digestion
      • Ligation with T4 DNA Ligase
    • Multi-Fragment Assembly
      • Golden Gate
      • LCR
      • Gibson Assembly
      • TA Cloning
    • Genome Editing
      • Lambda Red Recombination
      • MAGE
      • pORTMAGE
      • CRISPR/Cas9
    • Transformation and Integration
      • E. coli Electroporation
      • E. coli Heat Shock
      • B. subtilis
  • Test
    • DNA
      • Gel Electrophoresis
      • Sanger Sequencing
      • Illumina
      • LCR-Seq
    • Protein
      • Flow Cytometry
      • Fluorescence Activated Cell Sorting
      • Gram's Iodine Stain
      • LacZ (beta-gal)
      • Microplate Fluoresence
      • Neurosporene
      • SDS-PAGE
      • TX-TL Crude Cell Extract Prep
      • Transcription-Translation (TX-TL)
    • RNA
      • Spin Column-Based Extraction
      • Phenol-Chloroform Extraction
      • Reverse Transcriptase (RT)
      • qPCR with TaqMan
      • qPCR with SYBR Green
      • Targeted RNA-Seq
      • Transcriptome RNA-Seq
      • T7 HiScribe Kit RNA Synthesis
      • T7 RNAP Reaction Clean-Up
    • Metabolite
      • HPLC
      • LC-MS
    • Other
      • TCSPC
      • MinION
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  1. Modeling & Algorithms

Linux

The Linux operating systems are fundamental to the world of scientific computing. Almost all major scientific tools across all scientific disciplines are developed and maintained in Linux. Scientists routinely execute their tools and analyses in a Linux environment. Part of this is because Linux is a solidly engineered operating system, is robust, and free. At Salis Lab we always adhere to the most cutting edge tools, and thus must conform to the Linux philosophy or open-source software and wide-spread collaboration.

In this part of the book, we introduce you how to install a Linux OS and how to use the BASH (Bourne-Again Shell) to perform basic tasks. These will include creating (C), reading (R), updating (U) and deleting (D) (collecively termed as CRUD operations) files and directories and navigating the file system. We will also demonstrate how to install some basic programs that will be immediately useful to you in your lab-work (for example, a modern text-editor). Finally, most of the open-sourced (or closed-sourced) software and analyses are usually stored in websites like GitHub that use the git protocol. For example, all of our code including this gitbook is hosted on GitHub. So, we will demonstrate how to use the git version control system to download a git repository and keep it updated from using our Private-Code hosted on GitHub as an example.

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Last updated 6 years ago