TCSPC

*This protocol needs to be completed.

Information on dyes and quenchers

  • Our TCSPC setup uses a 635 nm monochromatic LED laser. Any fluorescent dye that includes 635 nm light in its absorbance spectrum can be used in an experiment.

  • Absorbance, emission spectra of common dyes: https://www.chroma.com/spectra-viewer

  • (As of Jan 2017) IDT makes the following internally modified DNA oligos:

Dyes

On Catalogue

  • Cy5 (5’-3’)

Off Catalogue

  • Cy5 (NHS ester-linked)

  • Atto 647 (NHS ester-linked)

Quencher

Off Catalogue

  • Black Hole Quencher-2

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